| Expression pattern: |
DN |
| Associated gene: |
PRPF40B, TEP1, KEAP1, NRF2, ANXA1 |
| Associated microRNA: |
- |
| Biological function: |
Inhibits GSC proliferation, stemness, and invasion; reverses TMZ resistance by inducing ferroptosis; suppresses glioma progression in vivo; reduces TAM recruitment and M2 polarization via NRF2/ANXA1. |
| Molecular mechanism: |
circMAN1A2 directly binds TEP1, disrupts TEP1-KEAP1 interaction, promotes KEAP1-mediated NRF2 degradation, inducing ferroptosis; NRF2 transcriptionally upregulates ANXA1 to modulate TAM recruitment and M2 polarization; PRPF40B promotes circMAN1A2 biogenesis. |
| Biological pathway or process: |
ferroptosis (promotes); proliferation (inhibits); invasion (inhibits); stemness (inhibits); chemoresistance (inhibits); immune regulation (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Western Blot; Transfection; CCK8; EdU Staining; MTT; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; ROC Analysis |
| Clinical significance: |
Lower circMAN1A2 expression is associated with higher WHO grade and poorer prognosis; circMAN1A2 may serve as a biomarker for predicting survival prognosis in glioma patients. |
| Description: |
circMAN1A2 (hsa_circ_0000118) is down-regulated in glioma/GBM and low expression is associated with higher WHO grade and poorer survival. Functionally, circMAN1A2 suppresses GSC malignant phenotypes and increases TMZ sensitivity by inducing ferroptosis. Mechanistically, it binds TEP1 to promote KEAP1-mediated NRF2 degradation, reducing NRF2/ANXA1 signaling linked to TAM recruitment and M2 polarization. |
| Confidence score: |
0.8949 |