| Expression pattern: |
DN |
| Associated gene: |
HSP90 |
| Associated microRNA: |
- |
| Biological function: |
Induces ferroptosis and inhibits M2 polarization of tumor-associated macrophages, thereby suppressing prostate cancer cell invasion, migration, EMT, tumor growth and metastasis. |
| Molecular mechanism: |
circCCDC719-13 directly binds HSP90, promotes K48-linked ubiquitination and proteasomal degradation of HSP90, increasing ferroptosis (ROS/Fe2+/lipid peroxidation) and shifting TAMs toward M1 polarization; reduced M2 cytokines (TGF-beta, IL-10) attenuate EMT in prostate cancer cells. |
| Biological pathway or process: |
ferroptosis (promotes); EMT (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); macrophage polarization (other); immune regulation (other); ubiquitination (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Sanger Sequencing; Clinical Sample Validation; Transfection; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; ELISA; Western Blot; Co-IP; Bioinformatics Analysis; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Transwell Assay; Wound Healing Assay |
| Clinical significance: |
circCCDC719-13 is downregulated in TAMs in prostate cancer tissues and is associated with prostate cancer metastasis. |
| Description: |
circCCDC719-13 is downregulated in prostate cancer-associated TAMs/M2 macrophages. Its overexpression drives ferroptosis and repolarizes TAMs toward an M1-like state by binding to HSP90 and promoting its ubiquitination/degradation, thereby reducing EMT-associated invasion/migration and suppressing tumor growth and metastasis in vivo. |
| Confidence score: |
0.7861 |