| Expression pattern: |
UP |
| Associated gene: |
TGM2 |
| Associated microRNA: |
miR-494-3p |
| Biological function: |
promotes MTX resistance, cell viability, migration and invasion; inhibits apoptosis; knockdown enhances MTX sensitivity and suppresses tumor growth |
| Molecular mechanism: |
ceRNA sponge: circ_0081001 sponges miR-494-3p to upregulate TGM2 |
| Biological pathway or process: |
apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); chemoresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
higher in MTX-resistant patients and distinguishes sensitive vs resistant tissues (ROC AUC 0.7796); high expression associated with poor survival |
| Description: |
circ_0081001 is up-regulated in osteosarcoma, especially in MTX-resistant tissues/cells, and high expression predicts poor survival. It promotes MTX resistance and malignant phenotypes by acting as a ceRNA sponge for miR-494-3p, thereby increasing TGM2 expression; circ_0081001 knockdown enhances MTX sensitivity in vitro and in xenograft tumors. |
| Confidence score: |
0.8066 |