| Expression pattern: |
UP |
| Associated gene: |
YBX1, E2F1 |
| Associated microRNA: |
- |
| Biological function: |
promotes breast cancer cell growth/proliferation and tumorigenesis |
| Molecular mechanism: |
binds YBX1 and inhibits ubiquitin/proteasome-dependent degradation of YBX1, thereby increasing YBX1-dependent E2F1 transcription |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ubiquitination (inhibits) |
| Detected method: |
Q
H
S
|
| Validation methods: |
circRNA-seq; Bioinformatics Analysis; RT-qPCR; ISH (In Situ Hybridization); Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Luciferase Reporter Assay; ChIP / ChIP-seq; Western Blot; Co-IP |
| Clinical significance: |
Higher expression in BC tissues and associated with malignant progression features (larger tumours, lymph node metastasis, high Ki67, ER- subgroup); correlation with YBX1 protein and E2F1 mRNA in BC tissues |
| Description: |
In breast cancer, hsa_circ_0007990 (circPGAP3) is up-regulated in tissues and cell lines and promotes tumor cell growth in vitro and in vivo. It predominantly localizes to the nucleus, binds YBX1, inhibits ubiquitin/proteasome-dependent degradation of YBX1, and thereby increases YBX1-dependent E2F1 transcription to drive proliferation/cell-cycle progression. |
| Confidence score: |
0.8647 |