| Expression pattern: |
UP |
| Associated gene: |
TPPP3, Ago2 |
| Associated microRNA: |
miR-1182 |
| Biological function: |
promotes autophagy and proliferation and inhibits apoptosis in ALL cells; supports tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circMUC16 sponges miR-1182 to relieve repression of TPPP3 |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circMUC16 is up-regulated in ALL cell lines and promotes malignant phenotypes by enhancing autophagy and proliferation while inhibiting apoptosis. Mechanistically, it functions as a ceRNA that sponges miR-1182 to increase TPPP3 expression, and circMUC16 knockdown suppresses tumor growth in a Jurkat xenograft model. |
| Confidence score: |
0.853 |