| Expression pattern: |
DN |
| Associated gene: |
PALM, Ago2 |
| Associated microRNA: |
miR-330-5p |
| Biological function: |
Inhibits cervical cancer cell proliferation, migration and invasion; promotes apoptosis; suppresses xenograft tumor growth. |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-330-5p to regulate PALM expression (circ-NOLC1/miR-330-5p/PALM axis). |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Transfection; CCK8; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Microarray |
| Clinical significance: |
Potential diagnostic biomarker and therapeutic target (clinical validation not performed). |
| Description: |
circ-NOLC1 (hsa_circ_0019686) is down-regulated in cervical cancer cell lines and acts as a tumor suppressor. It mainly localizes to the cytoplasm and functions as a ceRNA to sponge miR-330-5p, thereby increasing PALM expression and inhibiting proliferation/migration/invasion while promoting apoptosis, including suppression of xenograft tumor growth. |
| Confidence score: |
0.7893 |