circRNA basic information
circBase ID: hsa_circ_0046264
Name: hsa_circ_P4HB
Synonym: circ_0046264
Host Gene: P4HB
Genomic location(hg19): chr17:79813017-79817263:-
Genomic location(hg38): chr17:81855141-81859387:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

excised tumor tissues

Cell lines:

A549; H460

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UN
Associated gene: -
Associated microRNA: miR-522-3p
Biological function: circ_0046264 acts as a tumor suppressor in NSCLC cells; its overexpression inhibits cell viability, colony formation, adhesion, migration and invasion, while its knockdown partially counteracts bufalin-mediated anti-tumor effects.
Molecular mechanism: circ_0046264 directly targets and negatively regulates miR-522-3p, mediating the anti-tumor effect of bufalin through the circ_0046264/miR-522-3p axis.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model

Clinical significance:

-

Description:

This study identifies circ_0046264 as a bufalin-responsive tumor-suppressive circRNA in NSCLC cell models. Bufalin increases circ_0046264 expression, and circ_0046264 suppresses NSCLC proliferation, adhesion, migration, invasion and xenograft tumor growth by targeting miR-522-3p.

Confidence score:

0.6578

Other information
Title:

Bufalin inhibits the malignant development of non-small cell lung cancer by mediating the circ_0046264/miR-522-3p axis.

Journal: Biotechnology letters
Published: 2021
PubMed ID: 33534015
Study type:

biological research

Data availability: available from the corresponding author on reasonable request
Code availability: -