circRNA basic information
circBase ID: hsa_circ_0008305
Name: hsa_circ_PTK2
Synonym: circ-PTK2
Host Gene: PTK2
Genomic location(hg19): chr8:141799572-141840625:-
Genomic location(hg38): chr8:140789473-140830526:-
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008170
MONDO name: ovarian cancer
Disease details: ovarian cancer
Disease DO ID:
 2394
Disease MeSH ID:
 D010051
Disease NCIt ID:
 C7431
Disease ICD11 ID:
 685124533
Disease OMIM ID:
 167000
Species: Human
Species details: Homo sapiens
Tissue specimen:

ovarian cancer tissue; normal ovary tissue
Cell lines:

SK-OV-3; OVCAR-3; 293T; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FOXC1, CHD1L
Associated microRNA: miR-639
Biological function: promotes migration and invasion; promotes angiogenesis; promotes EMT; promotes in vivo tumor formation
Molecular mechanism: ceRNA mechanism: circ-PTK2 binds miR-639 and regulates FOXC1
Biological pathway or process:

migration (promotes); invasion (promotes); angiogenesis (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
H
S
Validation methods:

RNA-seq; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; RT-qPCR; Clinical Sample Validation; Transfection; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Luciferase Reporter Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

Circ-PTK2 expression was significantly higher in the ovarian cancer tissues compared with normal ovary tissues.
Description:

circ-PTK2 (hsa_circ_0008305) is up-regulated in ovarian cancer tissues and promotes ovarian cancer progression by enhancing migration, invasion, angiogenesis, EMT and in vivo tumor growth. Mechanistically, circ-PTK2 acts as a ceRNA by binding miR-639, thereby relieving miR-639-mediated repression of FOXC1.
Confidence score:

0.8256
Other information
Title:

circ-PTK2 (hsa_circ_0008305) regulates the pathogenic processes of ovarian cancer via miR-639 and FOXC1 regulatory cascade.

Journal: Cancer cell international
Published: 2021
PubMed ID: 34034740
Study type:

combined biological and clinical study
Data availability: The online version contains supplementary material available at 10.1186/s12935-021-01985-x.
Code availability: -