| Expression pattern: |
UP |
| Associated gene: |
BMI1, CCND1 |
| Associated microRNA: |
miR-15a-5p |
| Biological function: |
Promotes proliferation, migration, and invasion of gastric cancer cells in vitro. |
| Molecular mechanism: |
Likely acts via a ceRNA-like interaction by directly binding miR-15a-5p, consistent with modulation of miR-15a-5p target genes BMI1 and CCND1. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; divergent primers PCR; Transfection; MTT; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; Clinical Sample Validation; ROC Analysis; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-ANKRD36C expression was associated with shorter overall survival (OS) and was an independent prognostic biomarker in multivariate Cox regression (HR = 2.41). |
| Description: |
circ-ANKRD36C is up-regulated in gastric cancer tissues and promotes GC cell proliferation, migration, and invasion in vitro. It directly binds miR-15a-5p and is consistent with a ceRNA-like mechanism affecting miR-15a-5p targets (e.g., BMI1 and CCND1). High circ-ANKRD36C expression predicts poorer overall survival and serves as an independent prognostic biomarker. |
| Confidence score: |
0.8323 |