| Expression pattern: |
DN |
| Associated gene: |
ZC3H12C, N-cadherin, AGO2, CD8+ T cells |
| Associated microRNA: |
miR-576-5p |
| Biological function: |
Inhibits CRC metastasis/migration/invasion; downregulates N-cadherin; enhances CD8+ T cell infiltration and CD8+ T cell proliferation; suppresses tumor growth in vivo. |
| Molecular mechanism: |
ceRNA sponge of miR-576-5p to relieve repression of ZC3H12C, leading to reduced N-cadherin; associated with AGO2/RISC binding and cytoplasmic co-localization with miR-576-5p. |
| Biological pathway or process: |
ceRNA regulation (promotes); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); immune regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; Western Blot; Transwell Assay; Wound Healing Assay; RIP (RNA Immunoprecipitation); FISH / smFISH; Luciferase Reporter Assay; Clinical Sample Validation; Survival Analysis; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle）; CCK8; ELISA; Bioinformatics Analysis |
| Clinical significance: |
Low expression of circRNF216 is associated with lower overall survival in CRC; proposed as a potential biomarker/diagnostic marker and therapeutic target. |
| Description: |
circRNF216 (RNF216-derived exonic circRNA) is down-regulated in colorectal cancer and acts as a tumor suppressor. It sponges miR-576-5p to de-repress ZC3H12C, leading to reduced N-cadherin and inhibited migration/invasion/metastasis, and it also enhances CD8+ T-cell infiltration/proliferation via ZC3H12C, supporting potential diagnostic/therapeutic value. |
| Confidence score: |
0.8684 |