| Expression pattern: |
DN |
| Associated gene: |
FUS |
| Associated microRNA: |
hsa-miR-1265, hsa-miR-326 |
| Biological function: |
Inhibits LPS-induced DNA damage and inflammatory responses in hDPSCs; alleviates pulpitis-associated DNA damage and inflammation in vivo. |
| Molecular mechanism: |
circ_0002456 binds FUS and restricts its nuclear export, suppressing NF-kappaB activation to mitigate DNA damage and inflammatory cytokine expression. |
| Biological pathway or process: |
NF-kappaB (inhibits); inflammation (inhibits); apoptosis (not specified) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Western Blot; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Transfection; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
- |
| Description: |
circ_0002456 is down-regulated in pulpitis and in LPS-stimulated hDPSCs. It binds the RBP FUS, limits FUS nuclear export, and suppresses NF-kappaB activation, thereby reducing DNA damage (gamma-H2AX) and inflammatory cytokine expression; overexpression also attenuates these responses in a mouse pulpitis model. |
| Confidence score: |
0.7929 |