| Expression pattern: |
UP |
| Associated gene: |
YTHDF2, FUT8, mFUT8, AGO2, HRSP12, POP2 |
| Associated microRNA: |
miR-186-5p |
| Biological function: |
Promotes malignant progression, invasion, migration and metastasis of LUAD in a FUT8-dependent manner. |
| Molecular mechanism: |
m6A-modified circFUT8 is recognized by YTHDF2; circFUT8 competitively binds YTHDF2 to reduce YTHDF2 association with mFUT8 and stabilize FUT8 mRNA; circFUT8 also acts as a miRNA sponge for miR-186-5p to increase mFUT8 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); m6A modification (other); mRNA stability (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; divergent primers PCR; Sanger Sequencing; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); RNA Pull-Down; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
In LUAD, circFUT8 (hsa_circ_0003028) is up-regulated and mainly localized in the cytoplasm. It promotes malignant progression by stabilizing FUT8 mRNA via competitive interaction with the m6A reader YTHDF2 and by sponging miR-186-5p to increase FUT8 expression, thereby enhancing invasion and metastasis. |
| Confidence score: |
0.8624 |