| Expression pattern: |
UP |
| Associated gene: |
EIF4 A3, N-cadherin mRNA, Vimentin mRNA |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, migration, invasion and EMT in BUC |
| Molecular mechanism: |
circ_RPPH1 binds EIF4 A3 and prevents EIF4 A3 recruitment/enrichment on N-cadherin and Vimentin mRNAs, thereby promoting EMT |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); mRNA stability (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; Colony Formation Assay; CCK8; Wound Healing Assay; Transwell Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
High circ_RPPH1 levels in BUC correlated with tumor invasion depth. |
| Description: |
circ_RPPH1 is up-regulated in bladder urothelial carcinoma and promotes tumor cell proliferation, migration/invasion and EMT. Mechanistically, circ_RPPH1 binds the RBP EIF4A3 and reduces EIF4A3 enrichment on N-cadherin and Vimentin mRNAs, increasing EMT marker expression and driving EMT. |
| Confidence score: |
0.7045 |