| Expression pattern: |
DN |
| Associated gene: |
HELLS |
| Associated microRNA: |
miR-335-5p |
| Biological function: |
Alleviates mechanical overloading-induced chondrocyte senescence and cartilage degeneration; protects against OA progression. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-335-5p, thereby relieving miR-335-5p-mediated inhibition of HELLS and maintaining NHEJ DNA repair to reduce DNA damage and senescence. |
| Biological pathway or process: |
ceRNA regulation (promotes); mitochondrial function (not specified) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; divergent primers PCR; Sanger Sequencing; RNase R Treatment; Luciferase Reporter Assay; RNA Pull-Down; FISH / smFISH; RIP (RNA Immunoprecipitation); Transfection; Western Blot; In Vivo Animal Model; IF (Immunofluorescence); IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circKIAA0586 is down-regulated in OA cartilage and in mechanically overloaded human chondrocytes. It functions as a cytoplasmic sponge for miR-335-5p, thereby preserving HELLS expression and reducing DNA-damage-associated senescence and cartilage degeneration in OA models. |
| Confidence score: |
0.8565 |