| Expression pattern: |
UP |
| Associated gene: |
YBX1, CDC25A, CIAPIN1, XIAP, AKT, mTOR, HIF-1alpha, HK2, LDHA |
| Associated microRNA: |
- |
| Biological function: |
promotes survival and proliferation; inhibits apoptosis; promotes tumor growth and glycolysis-driven tumorigenesis |
| Molecular mechanism: |
binds to YBX1 and promotes YBX1 phosphorylation (S102), activating AKT/mTOR/HIF-1alpha signaling and upregulating glycolysis-related factors (HK2, LDHA, HIF-1alpha) |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); PI3K/AKT/mTOR (promotes); HIF-1 signaling (promotes); glycolysis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
B
H
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Northern Blot; FISH / smFISH; ISH (In Situ Hybridization); RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
BaseScope showed stage-specific elevation of circPOLD1 in NCE/LSIL/HSIL/CSCC; serum circPOLD1 increased progressively and ROC analysis supported diagnostic potential; circPOLD1 was significantly associated with lymph node metastasis |
| Description: |
circPOLD1 is progressively up-regulated from normal cervix to HSIL and CSCC and acts as an oncogenic circRNA. It directly binds the RBP YBX1, promotes YBX1 phosphorylation, and activates AKT/mTOR/HIF-1alpha-associated glycolysis to enhance cervical cancer cell proliferation and tumor growth; it also shows diagnostic potential in tissue (BaseScope) and serum (ROC). |
| Confidence score: |
0.8834 |