circRNA basic information
circBase ID: hsa_circ_0000514
Name: hsa_circ_RPPH1
Synonym: circRPPH1
Host Gene: RPPH1
Genomic location(hg19): chr14:20811305-20811436:-
Genomic location(hg38): chr14:20343146-20343277:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; surrounding normal tissues; xenograft tumors
Cell lines:

T47D; MCF-7; MDA-MB-231; BT549; MCF10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FOXP4, PCNA, MMP9, HK2
Associated microRNA: miR-296-5p
Biological function: circRPPH1 promotes breast cancer cell growth, migration, invasion, metastasis, glycolysis, and in vivo tumor growth; circRPPH1 knockdown suppresses these malignant phenotypes.
Molecular mechanism: circRPPH1 acts as a ceRNA by sponging miR-296-5p, thereby relieving miR-296-5p-mediated inhibition of FOXP4 and enhancing FOXP4 expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); glycolysis (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

RT-qPCR; Clinical Sample Validation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Survival Analysis
Clinical significance:

Up-regulation of circRPPH1 is closely correlated with poor prognosis, lymph node metastasis, and higher TNM stages in breast cancer patients; circRPPH1 may be a therapeutic target.
Description:

circRPPH1 is up-regulated in breast cancer tissues and cells and its high expression is associated with poor prognosis, lymph node metastasis, and higher TNM stage. Functionally, circRPPH1 promotes breast cancer cell proliferation, migration, invasion, glycolysis, and xenograft tumor growth. Mechanistically, circRPPH1 sponges miR-296-5p to increase FOXP4 expression, forming a circRPPH1/miR-296-5p/FOXP4 regulatory axis.
Confidence score:

0.8568
Other information
Title:

CircRPPH1 serves as a sponge for miR-296-5p to enhance progression of breast cancer by regulating FOXP4 expression.

Journal: American journal of translational research
Published: 2021
PubMed ID: 34377235
Study type:

combined biological and clinical study
Data availability: GSE101123
Code availability: -