| Expression pattern: |
UP |
| Associated gene: |
IGF2BP2, DTX3L mRNA |
| Associated microRNA: |
- |
| Biological function: |
promotes radioresistance; alleviates radiation-induced DNA damage; inhibits radiosensitivity; inhibits apoptosis under irradiation; alleviates radiation-induced proliferation inhibition |
| Molecular mechanism: |
circEYA3 binds the RBP IGF2BP2 and enhances IGF2BP2-mediated stabilization of DTX3L mRNA, reducing radiation-induced DNA damage. |
| Biological pathway or process: |
mitochondrial function (not specified) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Transfection; CCK8; Annexin V/PI Flow Cytometry; IF (Immunofluorescence); In Vivo Animal Model |
| Clinical significance: |
circEYA3 might be a predictive indicator and intervention option for 125I brachytherapy or external radiotherapy in HCC. |
| Description: |
In HCC treated with 125I seeds or X-ray irradiation, circEYA3 is upregulated and promotes radioresistance. It binds the RBP IGF2BP2 and enhances IGF2BP2-mediated stabilization of DTX3L mRNA, alleviating radiation-induced DNA damage and reducing apoptosis under irradiation. |
| Confidence score: |
0.7958 |