| Expression pattern: |
UP |
| Associated gene: |
p196, KHDRBS3, ULK1 mRNA, ULK1 |
| Associated microRNA: |
- |
| Biological function: |
Promotes HCC cell proliferation and invasion and enhances autophagy; exosome-delivered circFOXP1 enhances cancer cell progression. |
| Molecular mechanism: |
circFOXP1 encodes the protein p196; p196 binds KHDRBS3, forming a complex that binds/stabilizes ULK1 mRNA to increase ULK1 protein, thereby activating autophagy and promoting tumor progression; circFOXP1 is delivered via exosomes. |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); invasion (promotes); mRNA stability (promotes); ceRNA regulation (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Clinical Sample Validation; Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Cell Cycle Assay; In Vivo Animal Model; IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Western Blot; Actinomycin D / DRB Stability Assay; RNA-seq |
| Clinical significance: |
- |
| Description: |
circFOXP1 (hsa_circ_0008234) is up-regulated in HCC tissues and cell lines and can be transferred via exosomes. It encodes a 196-aa protein (p196) that binds KHDRBS3 to stabilize ULK1 mRNA, increasing ULK1 protein and autophagy, thereby promoting HCC cell proliferation and invasion and accelerating tumor progression. |
| Confidence score: |
0.8747 |