| Expression pattern: |
UP |
| Associated gene: |
YAP, CTGF, CCN1, MMP-9, MMP-13, IL-6 |
| Associated microRNA: |
- |
| Biological function: |
Promotes RA-FLS migration and invasion and inflammatory response (IL-6 secretion); increases MMP-9 and MMP-13 expression. |
| Molecular mechanism: |
Regulates Hippo-YAP signaling; circ_0002970 knockdown downregulates YAP and downstream CTGF and CCN1; Hippo-YAP inhibitor verteporfin reverses circ_0002970 overexpression effects. |
| Biological pathway or process: |
Hippo/YAP (promotes); migration (promotes); invasion (promotes); inflammation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; FISH / smFISH; RNase R Treatment; divergent primers PCR; Sanger Sequencing; Transfection; Transwell Assay; ELISA; Western Blot; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circ_0002970 is upregulated in RA fibroblast-like synoviocytes and promotes migration, invasion, and inflammatory IL-6 secretion. It acts by activating Hippo/YAP signaling and increasing YAP with downstream CTGF and CCN1 expression; inhibiting Hippo/YAP (verteporfin) reverses circ_0002970-driven phenotypes. |
| Confidence score: |
0.7589 |