| Expression pattern: |
DN |
| Associated gene: |
p-PDK1 |
| Associated microRNA: |
- |
| Biological function: |
Inhibits proliferation, radiation resistance, and in vivo tumorigenicity in GBM (via its encoded protein AKT3-174aa). |
| Molecular mechanism: |
circ-AKT3 encodes AKT3-174aa, which acts as a molecular decoy to competitively bind phosphorylated PDK1 and reduce AKT Thr308 phosphorylation, thereby dampening PI3K/AKT signaling. |
| Biological pathway or process: |
PI3K/AKT (inhibits); proliferation (inhibits); radioresistance (inhibits) |
| Detected method: |
Q
B
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; divergent primers PCR; Sanger Sequencing; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; EdU Staining; Colony Formation Assay; Cell Cycle Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis |
| Clinical significance: |
AKT3-174aa expression negatively correlated with GBM patient prognosis, suggesting potential prognostic marker utility (circ-AKT3 is the RNA encoding AKT3-174aa and is also low in GBM). |
| Description: |
circ-AKT3 (hsa_circ_0017250) is down-regulated in glioblastoma and is mainly cytoplasmic. It encodes the tumor-suppressive peptide AKT3-174aa, which binds p-PDK1 and reduces AKT Thr308 phosphorylation, thereby inhibiting PI3K/AKT signaling and malignant phenotypes such as proliferation, radioresistance, and in vivo tumorigenicity. |
| Confidence score: |
0.7941 |