| Expression pattern: |
UP |
| Associated gene: |
VEGFA, Ago2, RanGAP1 mRNA |
| Associated microRNA: |
miR-877-3p |
| Biological function: |
Promotes gastric cancer cell proliferation, invasion, migration, tumor growth, metastasis and angiogenesis; plasma exosomal circ-RanGAP1 enhances GC cell invasion and migration and may serve as a biomarker. |
| Molecular mechanism: |
circ-RanGAP1 acts as a ceRNA/miRNA sponge for miR-877-3p, relieving repression of VEGFA and increasing VEGFA expression; exosomal circ-RanGAP1 may mediate intercellular transfer that enhances invasion and migration. |
| Biological pathway or process: |
ceRNA regulation (promotes); VEGF/VEGFR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; ROC Analysis; Cohort Study |
| Clinical significance: |
High circ-RanGAP1 expression is associated with tumor size, lymph node metastasis, advanced clinical stage and poor overall survival; combined circ-RanGAP1 and VEGFA detection provides more accurate prognosis; plasma exosomal circ-RanGAP1 may serve as a promising biomarker for GC patients. |
| Description: |
circ-RanGAP1/hsa_circ_0063526 is upregulated in gastric cancer tissues and plasma exosomes and high expression predicts poor overall survival. Functionally, it promotes GC proliferation, invasion, migration, angiogenesis, tumor growth and metastasis. Mechanistically, circ-RanGAP1 acts as a ceRNA for miR-877-3p to increase VEGFA expression, and plasma exosomal circ-RanGAP1 may contribute to GC progression and biomarker utility. |
| Confidence score: |
0.874 |