| Expression pattern: |
UP |
| Associated gene: |
HMGA2 |
| Associated microRNA: |
miR-1224-5p |
| Biological function: |
Promotes proliferation, migration, invasion and EMT, and inhibits apoptosis in breast cancer cells; enhances xenograft tumor growth. |
| Molecular mechanism: |
Acts as a ceRNA (molecular sponge) for miR-1224-5p to upregulate HMGA2, thereby promoting malignant phenotypes including EMT. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis |
| Clinical significance: |
Upregulated circPOLA2 in BC is associated with poor prognosis; high circPOLA2 expression correlates with TNM stage, tumor size, and distant metastasis, and predicts shorter overall survival. |
| Description: |
circPOLA2 is upregulated in breast cancer tissues and cell lines and promotes malignant phenotypes (proliferation, migration, invasion, EMT) while inhibiting apoptosis. Mechanistically, circPOLA2 functions as a ceRNA that sponges miR-1224-5p to increase HMGA2 expression, and circPOLA2 knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.8534 |