circRNA basic information
circBase ID: hsa_circ_0004585
Name: -
Synonym: circ_0004585
Host Gene: -
Genomic location(hg19): chr15:81166204-81212640:+
Genomic location(hg38): chr15:80873863-80920299:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor specimens; paracancerous normal tissues; 5-FU-sensitive cancer tissues; 5-FU-resistant cancer tissues
Cell lines:

HCT116; HCT8; HCT116/FU; HCT8/FU; NCM460; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CCND1
Associated microRNA: miR-874-3p
Biological function: circ_0004585 promotes CRC cell viability, proliferation, migration, invasion, tube formation/angiogenesis, tumor growth, and 5-FU resistance, while inhibiting apoptosis; knockdown enhances chemosensitivity to 5-FU.
Molecular mechanism: circ_0004585 acts as a ceRNA by sponging miR-874-3p, thereby positively regulating CCND1 and promoting CRC progression and 5-FU resistance.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); chemoresistance (promotes); ceRNA regulation (other); metastasis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model
Clinical significance:

circ_0004585 is highly expressed in CRC tissues and especially in 5-FU-resistant cancer tissues, suggesting it may serve as a target for CRC treatment and drug resistance.
Description:

circ_0004585 is up-regulated in CRC tissues and cells, with even higher expression in 5-FU-resistant CRC samples and cell lines. Functionally, circ_0004585 promotes malignant phenotypes and 5-FU resistance, while its knockdown suppresses proliferation, migration, invasion, angiogenic tube formation, and tumor growth and promotes apoptosis. Mechanistically, circ_0004585 acts through the miR-874-3p/CCND1 axis to regulate CRC progression and chemoresistance.
Confidence score:

0.6544
Other information
Title:

Knockdown of circ_0004585 enhances the chemosensitivity of colorectal cancer cells to 5-fluorouracil via the miR-874-3p/CCND1 axis.

Journal: Histology and histopathology
Published: 2023
PubMed ID: 35900059
Study type:

combined biological and clinical study
Data availability: Please contact the correspondence author for the data request.
Code availability: -