| Expression pattern: |
DN |
| Associated gene: |
PTEN |
| Associated microRNA: |
miR-181b-5p |
| Biological function: |
Inhibits hepatic stellate cell activation and proliferation; suppresses alpha-SMA and type I collagen expression (anti-fibrotic effect). |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-181b-5p, thereby promoting PTEN expression. |
| Biological pathway or process: |
proliferation (inhibits); fibrosis (inhibits); ceRNA regulation (promotes); PI3K/AKT (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Liver cMTO1 differentiated liver cirrhosis patients from healthy controls (AUC 0.959). |
| Description: |
cMTO1 is down-regulated in cirrhotic/fibrotic liver and activated HSCs, and its restoration suppresses HSC proliferation and activation markers. Mechanistically, cMTO1 sponges miR-181b-5p in the cytoplasm to relieve repression of PTEN, thereby inhibiting fibrotic activation (via the PTEN-related PI3K/Akt context). |
| Confidence score: |
0.8389 |