circRNA basic information
circBase ID: hsa_circ_0005615
Name: hsa_circ_NFATC3
Synonym: circ_0005615 / circNFATC3
Host Gene: NFATC3
Genomic location(hg19): chr16:68155889-68157024:+
Genomic location(hg38): chr16:68121986-68123121:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0002974
MONDO name: cervical cancer
Disease details: cervical cancer / CC
Disease DO ID:
4362
Disease MeSH ID:
-
Disease NCIt ID:
C9311
Disease ICD11 ID:
1256072522
Disease OMIM ID:
603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tissues; adjacent nontumor tissues; tumors

Cell lines:

SiHa; CaSki; 293T; Ect1/E6E7

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: KDM2A, NFATC3, PCNA, MMP9, Bcl-2, Bax
Associated microRNA: miR-138-5p
Biological function: promotes cervical cancer cell proliferation, migration, invasion, metastasis and tumor growth; inhibits apoptosis
Molecular mechanism: circ_0005615 acts as a ceRNA by sponging miR-138-5p to positively regulate KDM2A expression
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)

Clinical significance:

circ_0005615 may be a potential target for the treatment of CC

Description:

circ_0005615 is up-regulated in cervical cancer tissues and cells and functions as an oncogenic circRNA. It promotes CC cell growth, migration, invasion, metastasis and xenograft tumor growth while suppressing apoptosis. Mechanistically, circ_0005615 sponges miR-138-5p to increase KDM2A expression, forming a circ_0005615/miR-138-5p/KDM2A regulatory axis that may represent a therapeutic target.

Confidence score:

0.844

Other information
Title:

Circ_0005615 promotes cervical cancer cell growth and metastasis by modulating the miR-138-5p/KDM2A axis.

Journal: Journal of biochemical and molecular toxicology
Published: 2023
PubMed ID: 37393518
Study type:

combined biological and clinical study

Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -