| Expression pattern: |
UP |
| Associated gene: |
PAX6 |
| Associated microRNA: |
miR-132-3p |
| Biological function: |
Promotes RB cell proliferation, migration and invasion; inhibits apoptosis; promotes tumor growth in vivo; exosomal circRNF20 promotes malignant behaviors of RB cells. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-132-3p to regulate PAX6 (ceRNA mechanism); exosomal transfer increases circRNF20 level in recipient RB cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Clinical Sample Validation |
| Clinical significance: |
circRNF20 elevation was linked to advanced TNM stage and poor overall survival rate; exosomal circRNF20 in serums might be a diagnostic marker for RB. |
| Description: |
circRNF20 is up-regulated in retinoblastoma tissues/cells and is associated with advanced TNM stage and worse overall survival. Functionally, circRNF20 promotes RB cell proliferation, migration/invasion and EMT while inhibiting apoptosis, and it enhances tumor growth in a xenograft model. Mechanistically, circRNF20 sponges miR-132-3p to upregulate PAX6, and circRNF20 is also present in patient serum-derived exosomes that can enhance malignant phenotypes in RB cells. |
| Confidence score: |
0.8616 |