| Expression pattern: |
UP |
| Associated gene: |
GNAS |
| Associated microRNA: |
miR-136-5p |
| Biological function: |
Promotes bladder cancer cell proliferation, migration and tumor growth; contributes to growth and metastasis; knockdown restrains proliferation, migration and in vivo tumorigenesis. |
| Molecular mechanism: |
circSPECC1 acts as a cytoplasmic miRNA sponge for miR-136-5p and promotes GNAS mRNA and protein expression via the circSPECC1/miR-136-5p/GNAS axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Increased expression of circSPECC1 correlates with larger tumor size and shorter overall survival time, and it may become a non-invasive biomarker for diagnosis and therapeutic target for treatment. |
| Description: |
circSPECC1/circ_0000745 is a SPECC1-derived circRNA that is up-regulated in bladder cancer tissues and cell lines. It functions as an oncogenic cytoplasmic ceRNA by sponging miR-136-5p, thereby increasing GNAS expression and promoting bladder cancer proliferation, migration, and in vivo tumor growth. High circSPECC1 expression is associated with larger tumor size and shorter overall survival. |
| Confidence score: |
0.817 |