circRNA basic information
circBase ID: hsa_circ_0123778
Name: hsa_circ_CTNNB1
Synonym: circ-CTNNB1
Host Gene: CTNNB1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

cancer tissues; peritumor tissues; tumor tissues; gastric cancer tissues; prostate cancer tissues; colon cancer tissues; primary gastric cancer tissues; paired adjacent normal tissues
Cell lines:

HEK293T; MKN-45; AGS; HeLa; PC-3; MCF-7; HCT-116; LoVo; HepG2; BE(2)-C; SH-SY5Y
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: DDX3, YY1, CTNNB1, b-catenin, WNT1, WNT3, AXIN2, FZD10, BMP4, p300, EZH2, HDAC1
Associated microRNA: -
Biological function: Circ-CTNNB1 promotes b-catenin activation, cancer cell viability, anchorage-independent growth, invasion, tumor growth, angiogenesis, lung metastasis, tumorigenesis, and aggressiveness; high expression is associated with poor overall survival.
Molecular mechanism: Circ-CTNNB1 binds DDX3, facilitates DDX3 interaction with YY1, increases YY1 transactivation, alters transcription of Wnt/b-catenin-related target genes, and promotes b-catenin activation and cancer progression.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes); other pathway/process (promotes)
Detected method:
Q
B
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; Northern Blot; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Circ-CTNNB1 was associated with unfavorable outcomes in patients with cancer, and high circ-CTNNB1 expression was associated with poor overall survival of gastric cancer patients.
Description:

Circ-CTNNB1 is a human CTNNB1-derived intronic circRNA that is upregulated in multiple cancer tissues and cell lines and predicts poor overall survival in gastric cancer. It predominantly localizes in the nucleus, binds DDX3, promotes DDX3-YY1 interaction and YY1 transactivation, increases Wnt/b-catenin-related target gene expression, and drives cancer growth, invasion, metastasis, angiogenesis, and aggressiveness.
Confidence score:

0.8957
Other information
Title:

Cis-Acting circ-CTNNB1 Promotes beta-Catenin Signaling and Cancer Progression via DDX3-Mediated Transactivation of YY1.

Journal: Cancer research
Published: 2019
PubMed ID: 30563889
Study type:

combined biological and clinical study
Data availability: GSE120496; Supplementary Table S1; Supplementary Table S2; Supplementary Table S3; Supplementary Table S4; Supplementary Table S5
Code availability: -