| Expression pattern: |
UP |
| Associated gene: |
CKAP2L, AGO2 |
| Associated microRNA: |
miR-647 |
| Biological function: |
Promotes glioblastoma cell proliferation, invasion, and glycolysis; promotes NHA proliferation via exosomal transfer. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-647 to upregulate CKAP2L; exosomal transfer mediates intercellular communication. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); RNA-seq |
| Clinical significance: |
High circ_0001583 expression significantly correlated with poor overall survival; high expression correlated with advanced WHO grade and presence of MRI-detected necrosis. |
| Description: |
circ_0001583 is upregulated in glioblastoma and is mainly localized in the cytoplasm. It promotes GBM cell proliferation, invasion and glycolysis by sponging miR-647 and relieving repression of CKAP2L, and can be transferred via exosomes to normal astrocytes to enhance their proliferation. |
| Confidence score: |
0.8556 |