circRNA basic information
circBase ID: hsa_circ_0057010
Name: hsa_circ_SP5
Synonym: circSP5
Host Gene: SP5
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; adjacent normal tissues; liver
Cell lines:

FHC; KM12SM; KM12; SW480; RKO; 293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SP5, BAMBI, Ago2
Associated microRNA: miR-1249-3p
Biological function: promotes liver metastasis of CRC; promotes CRC cell migration, invasion, and EMT; circSP5 depletion suppresses CRC metastasis in vitro and in vivo
Molecular mechanism: circSP5 acts as a ceRNA to sponge miR-1249-3p, enhances SP5 expression, and promotes SP5-mediated transcriptional activation of BAMBI
Biological pathway or process:

ceRNA regulation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); proliferation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis
Clinical significance:

circSP5 may provide a novel biomarker for treating CRC patients with liver metastasis
Description:

circSP5 is up-regulated in CRC, especially in CRC with liver metastasis, and promotes CRC migration, invasion, EMT, tumor growth, and liver metastasis. Mechanistically, circSP5 sponges miR-1249-3p to enhance SP5 expression, enabling SP5-mediated transcriptional activation of BAMBI. The study suggests circSP5 as a potential biomarker and therapeutic mechanism for CRC liver metastasis.
Confidence score:

0.8185
Other information
Title:

Circular RNA circSP5 promotes liver metastasis of colorectal cancer via SP5-mediated BAMBI transcription.

Journal: Functional & integrative genomics
Published: 2023
PubMed ID: 37596430
Study type:

combined biological and clinical study
Data availability: Research data can be obtained from the corresponding author if necessary; supplementary material available at https:// doi. org/ 10. 1007/ s10142-023-01142-9
Code availability: -