| Expression pattern: |
UP |
| Associated gene: |
HDAC6 |
| Associated microRNA: |
miR-433-3p |
| Biological function: |
promotes neuronal damage; promotes apoptosis and autophagy; knockdown alleviates PD-like symptoms and neuronal damage |
| Molecular mechanism: |
ceRNA sponge mechanism: circPRDM5 sponges miR-433-3p to regulate HDAC6 expression, affecting apoptosis and autophagy |
| Biological pathway or process: |
apoptosis (promotes); autophagy (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; Transfection; Luciferase Reporter Assay; RNA Pull-Down; CCK8; LDH; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; TUNEL; IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circPRDM5 is up-regulated in PD patient blood and in toxin-induced PD models. It mainly localizes to the cytoplasm and acts as a miRNA sponge for miR-433-3p, thereby increasing HDAC6 expression and promoting neuronal injury with increased apoptosis and autophagy; circPRDM5 knockdown is protective in SH-SY5Y cells and MPTP mice. |
| Confidence score: |
0.8635 |