| Expression pattern: |
UP |
| Associated gene: |
CREB1 |
| Associated microRNA: |
miR-384 |
| Biological function: |
promotes GC cell proliferation, migration and invasion; promotes cell cycle progression; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circ_0043947 sponges miR-384 to upregulate CREB1 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Back-Splice Junction PCR / divergent primers PCR; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high circ_0043947 expression was significantly related to the low overall survival of GC patients; correlated with advanced TNM stage, lymph node metastasis and distant metastasis |
| Description: |
Circ_0043947 is up-regulated in gastric cancer tissues and cells and functions as an oncogenic circRNA. It sponges miR-384 to increase CREB1 expression, thereby promoting proliferation, migration/invasion, cell-cycle progression and tumor growth while suppressing apoptosis; high expression is associated with worse overall survival. |
| Confidence score: |
0.8593 |