| Expression pattern: |
DN |
| Associated gene: |
COL4A4, caveolin-1 |
| Associated microRNA: |
- |
| Biological function: |
maintains blood-brain barrier integrity, enhances endothelial integrity and survival, reduces endothelial apoptosis, promotes endothelial migration, and preserves tight junction proteins after hypoxia/reoxygenation or ischemic stroke. |
| Molecular mechanism: |
Hypoxic neurons transfer CircOGDH to endothelial cells via small extracellular vesicles, which increases COL4A4 expression and thereby maintains tight junction proteins and vascular stability/BBB integrity; effects on permeability may also involve endothelial caveolae/transcytosis. |
| Biological pathway or process: |
extracellular vesicle (other); migration (promotes); apoptosis (inhibits); invasion (other); ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Clinical Sample Validation; Cohort Study; Bioinformatics Analysis; RNA-seq; Transfection; Western Blot; Annexin V/PI Flow Cytometry; Wound Healing Assay; In Vivo Animal Model; IF (Immunofluorescence) |
| Clinical significance: |
Lower plasma CircOGDH is associated with hemorrhagic transformation and larger hematoma volume in acute ischemic stroke; CircOGDH may serve as a biomarker for predicting hemorrhagic risk. |
| Description: |
CircOGDH is reduced in plasma of AIS patients with hemorrhagic transformation and negatively correlates with hematoma volume, suggesting a protective association with BBB integrity. Mechanistically, hypoxic neurons package and deliver CircOGDH via small extracellular vesicles to brain endothelial cells, where CircOGDH increases COL4A4 and helps preserve tight junction proteins, endothelial survival, migration, and barrier integrity after hypoxia/reoxygenation and in MCAO/R models. |
| Confidence score: |
0.6915 |