| Expression pattern: |
UP |
| Associated gene: |
ROBO1 |
| Associated microRNA: |
miR-197-3p |
| Biological function: |
circ_USP36 promotes ox-LDL-induced endothelial cell dysfunction by suppressing proliferation and cell cycle progression and inducing apoptosis, inflammation, and cell death; circ_USP36 silencing attenuates these effects. |
| Molecular mechanism: |
circ_USP36 functions as a miR-197-3p sponge and positively regulates ROBO1 expression through the miR-197-3p/ROBO1 axis. |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); apoptosis (promotes); inflammation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; EdU Staining; MTT; Western Blot; Cell Cycle Assay; Annexin V/PI Flow Cytometry; ELISA; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
circ_USP36 was highly expressed in serum samples of AS patients, suggesting potential involvement in AS progression; its association with clinicopathologic features was not analyzed. |
| Description: |
circ_USP36 is up-regulated in serum from AS patients and in ox-LDL-induced HUVECs. Functionally, circ_USP36 promotes endothelial dysfunction by inhibiting proliferation and cell cycle progression while enhancing apoptosis, inflammation, and cell death. Mechanistically, it acts as a miR-197-3p sponge to increase ROBO1 expression, forming a circ_USP36/miR-197-3p/ROBO1 regulatory axis. |
| Confidence score: |
0.7595 |