circRNA basic information
circBase ID: hsa_circ_0042881
Name: hsa_circ_NF1
Synonym: circ_0042881 / circNF1
Host Gene: NF1
Genomic location(hg19): chr17:29483000-29509683:+
Genomic location(hg38): chr17:31155982-31182665:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; adjacent non-tumor tissue; plasma
Cell lines:

MDA-MB-231; MCF-7; MDA-MB-415; T47D; ZR-75-1; BT474; AU565; SK-BR-3; HCC1143; CAL51; BT549; HCC70; MCF-10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SOS1, RAS, EIF4A3, AGO2
Associated microRNA: miR-217
Biological function: promotes proliferation, migration, invasion, tumor growth and metastasis in breast cancer
Molecular mechanism: circ_0042881 acts as a ceRNA by sponging miR-217, relieving repression of SOS1, activating RAS and downstream MEK/ERK and PI3K/AKT signaling; EIF4A3 promotes circ_0042881 circularization
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ERK (promotes); PI3K/AKT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; ROC Analysis; RNA-seq; Bioinformatics Analysis
Clinical significance:

circ_0042881 was elevated in BC tissues and plasma, associated with clinicopathological features, and showed diagnostic value by ROC (AUC = 0.802)
Description:

circ_0042881 (circNF1) is upregulated in breast cancer tissues and plasma and promotes proliferation, migration, invasion, tumor growth, and lung metastasis. It mainly localizes to the cytoplasm and acts as a ceRNA by sponging miR-217 to upregulate SOS1, thereby activating RAS and downstream MEK/ERK and PI3K/AKT signaling; EIF4A3 enhances its biogenesis.
Confidence score:

0.8711
Other information
Title:

EIF4A3-mediated circ_0042881 activates the RAS pathway via miR-217/SOS1 axis to facilitate breast cancer progression.

Journal: Cell death & disease
Published: 2023
PubMed ID: 37626035
Study type:

combined biological and clinical study
Data availability: GSE234870
Code availability: -