| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
miR-492 |
| Biological function: |
inhibits proliferation, migration, and invasion; promotes apoptosis; affects cell cycle progression (G0/G1 arrest upon overexpression) |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-492 (cytoplasmic co-localization; luciferase-validated binding) and regulates NSCLC cell phenotypes via miR-492. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; ROC Analysis; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0006423 has diagnostic value as a non-invasive biomarker for NSCLC (ROC AUC up to 0.992 in combined analysis). |
| Description: |
hsa_circ_0006423 (BCAR3-derived) is down-regulated in NSCLC plasma, tumors, and NSCLC cell lines and shows diagnostic potential by ROC analysis. Functionally, it acts as a cytoplasmic sponge of miR-492 and suppresses NSCLC cell proliferation, migration, and invasion while promoting apoptosis and inducing G0/G1 cell-cycle arrest. |
| Confidence score: |
0.8162 |