| Expression pattern: |
UP |
| Associated gene: |
IGF2 |
| Associated microRNA: |
miR-138-5p |
| Biological function: |
circ-FNDC3B acts as a carcinogenic circRNA in renal carcinoma, promotes cell proliferation and colony formation, inhibits apoptosis, and attenuates curcumin-mediated antitumor effects. |
| Molecular mechanism: |
circ-FNDC3B functions through a ceRNA mechanism by binding miR-138-5p and positively regulating IGF2; curcumin suppresses renal carcinoma tumorigenesis by decreasing circ-FNDC3B and inhibiting the circ-FNDC3B/miR-138-5p/IGF2 axis. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other); drug resistance (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circ-FNDC3B is up-regulated in renal carcinoma tissues and cell lines and promotes tumorigenic phenotypes. Mechanistically, it sponges miR-138-5p to enhance IGF2 expression, thereby supporting proliferation and suppressing apoptosis. Curcumin down-regulates circ-FNDC3B, and circ-FNDC3B overexpression weakens curcumin-induced inhibition of renal carcinoma growth in vitro and in xenografts. |
| Confidence score: |
0.7177 |