| Expression pattern: |
DN |
| Associated gene: |
SRSF2, PLCE1 |
| Associated microRNA: |
- |
| Biological function: |
promotes CRC cell proliferation, migration and invasion; inhibits apoptosis; promotes tumour growth in vivo |
| Molecular mechanism: |
circPLCE1 directly binds SRSF2 in the nucleus and represses SRSF2-dependent PLCE1 pre-RNA splicing, reducing PLCE1 mRNA production |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); mRNA stability (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
High circPLCE1 expression was significantly correlated with deeper invasion, more lymph node metastasis and advanced TNM stage. |
| Description: |
circPLCE1 (hsa_circ_0019230), derived from PLCE1, is down-regulated in CRC tissues but functions as a tumour promoter. It mainly localizes to the nucleus and binds SRSF2 to repress SRSF2-dependent PLCE1 pre-RNA splicing, reducing PLCE1 mRNA/protein and promoting malignant phenotypes and xenograft growth. |
| Confidence score: |
0.7536 |