| Expression pattern: |
DN |
| Associated gene: |
TEAD1, YTHDC1, Ago2 |
| Associated microRNA: |
miR‐219a‐2‐3p |
| Biological function: |
inhibits Hcy-mediated lipid accumulation in foam cells and attenuates atherosclerosis |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-219a-2-3p to regulate TEAD1 and activate AMPK signaling; Hcy induces m6A modification and YTHDC1-mediated nuclear export affecting sponge function |
| Biological pathway or process: |
AMPK (inhibits); proliferation (not specified); migration (not specified); invasion (not specified); apoptosis (not specified); ceRNA regulation (other); lipid metabolism (other pathway/process) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; MeRIP / MeRIP-seq; Transfection; Western Blot; Oil Red O Staining; In Vivo Animal Model; H&E Staining; IF (Immunofluorescence) |
| Clinical significance: |
circ-PIAS1-5 expression in blood is decreased in HHcy-associated atherosclerosis patients and shows diagnostic value by ROC analysis |
| Description: |
circ-PIAS1-5 is down-regulated by homocysteine in foam cells and HHcy-associated atherosclerosis and suppresses lipid accumulation and plaque formation. It functions as a ceRNA by sponging miR-219a-2-3p to upregulate TEAD1 and modulate AMPK signaling, while Hcy-driven m6A modification enables YTHDC1-dependent nucleo-cytoplasmic trafficking that impacts its sponge activity. Circulating/blood circ-PIAS1-5 shows potential diagnostic value for HHcy-associated atherosclerosis. |
| Confidence score: |
0.887 |