| Expression pattern: |
UP |
| Associated gene: |
cyclin-dependent kinase 4 / CDK4 |
| Associated microRNA: |
miR-198 |
| Biological function: |
promotes HCC cell proliferation, migration and invasion; promotes xenograft tumor growth |
| Molecular mechanism: |
ceRNA mechanism: circSP3 sponges miR-198 to de-repress/upregulate CDK4 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; divergent primers PCR; Back-Splice Junction PCR; Luciferase Reporter Assay; FISH / smFISH; Transfection; CCK8; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
circSP3 levels correlated with the tumor size and tumor-node-metastasis (TNM) stage; suggested as a biomarker for HCC |
| Description: |
circSP3 is up-regulated in hepatocellular carcinoma and promotes tumor cell proliferation, migration/invasion, and xenograft growth. Mechanistically, circSP3 functions as a cytoplasmic sponge for miR-198, thereby de-repressing CDK4 expression to drive HCC progression. |
| Confidence score: |
0.8266 |