| Expression pattern: |
UP |
| Associated gene: |
PDL1, UHRF1, PCNA, Bcl-2, C-caspase 3/t-caspase 3, MMP2, MMP9, IFN-gamma, TNF-alpha |
| Associated microRNA: |
miR-223-3p |
| Biological function: |
Promotes HCC cell proliferation, migration, invasion, angiogenesis and tumor growth; inhibits apoptosis; inhibits NK cell functions and contributes to NK cell exhaustion. |
| Molecular mechanism: |
hsa_circ_0048674 acts as a sponge for miR-223-3p to positively regulate PDL1 expression, thereby promoting malignant HCC phenotypes and NK cell function impairment. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); metastasis (promotes); immune regulation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); ELISA; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0048674 level was related to tumor size, TNM stages and lymph node metastasis; lower hsa_circ_0048674 level was observed in patients with higher NK cell proportion in blood. |
| Description: |
hsa_circ_0048674 is up-regulated in HCC tissues and cell lines and is associated with tumor size, TNM stage and lymph node metastasis. It promotes HCC progression by acting as a miR-223-3p sponge to increase PDL1 expression, thereby enhancing proliferation, migration, invasion, angiogenesis and tumor growth while suppressing apoptosis. It also impairs NK cell functions, suggesting an immune regulatory role in HCC. |
| Confidence score: |
0.8443 |