circRNA basic information
circBase ID: hsa_circ_0005890
Name: hsa_circ_GLIS3
Synonym: CircGLIS3 / circ_0005890
Host Gene: GLIS3
Genomic location(hg19): chr9:4191785-4286523:-
Genomic location(hg38): chr9:4191785-4286523:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma / GBM
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

U251; LN229; NHAs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: CAPG, GLIS3, Ago2
Associated microRNA: miR-449c-5p
Biological function: promotes proliferation; inhibits apoptosis
Molecular mechanism: circGLIS3 sponges miR-449c-5p to positively regulate CAPG and GLIS3 expression (ceRNA).
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; TUNEL; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

circGLIS3 (circ_0005890), derived from GLIS3, is up-regulated in glioblastoma cell lines and promotes proliferation while inhibiting apoptosis. Mechanistically, circGLIS3 mainly localizes to the cytoplasm and acts as a ceRNA by sponging miR-449c-5p, thereby upregulating CAPG and GLIS3.
Confidence score:

0.809
Other information
Title:

A circular RNA derived from GLIS3 accelerates the proliferation of glioblastoma cells through competitively binding with miR-449c-5p to upregulate CAPG and GLIS3.

Journal: BMC neuroscience
Published: 2022
PubMed ID: 36114444
Study type:

biological research
Data availability: 10.1186/s12868-022-00736-6
Code availability: -