| Expression pattern: |
UP |
| Associated gene: |
FOXO1, VEGF |
| Associated microRNA: |
miR-149-5p |
| Biological function: |
promotes VEGF production and angiogenesis in RA-FLS; its knockdown inhibits RA-FLS-induced angiogenesis and alleviates arthritis severity in CIA mice |
| Molecular mechanism: |
ceRNA/miRNA sponge: circHIPK3 sponges miR-149-5p to increase FOXO1 and downstream VEGF, forming a circHIPK3/miR-149-5p/FOXO1/VEGF functional module; ATO downregulates circHIPK3 to inhibit this module |
| Biological pathway or process: |
angiogenesis (promotes); migration (promotes); proliferation (other); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; ELISA; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis; Sanger Sequencing; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circHIPK3 is up-regulated in TNF-alpha-induced RA-FLS and promotes synovial angiogenesis by acting as a miR-149-5p sponge to increase FOXO1 and downstream VEGF. Arsenic trioxide suppresses this circHIPK3/miR-149-5p/FOXO1/VEGF module, and circHIPK3 knockdown alleviates angiogenesis and arthritis severity in CIA mice. |
| Confidence score: |
0.8442 |