| Expression pattern: |
UP |
| Associated gene: |
RPN2 |
| Associated microRNA: |
miR-1258 |
| Biological function: |
promotes HCC cell proliferation, migration, invasion and glycolysis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circ_0046599 sponges miR-1258 to increase RPN2 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Clinical Sample Validation; Survival Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
HCC patients with high circ_0046599 expression had lower overall survival than those with low circ_0046599 expression |
| Description: |
circ_0046599 is up-regulated in HCC tissues and cell lines and is mainly localized in the cytoplasm. It promotes HCC malignant phenotypes (proliferation, migration/invasion and glycolysis) and tumor growth by sponging miR-1258 to increase RPN2 expression. |
| Confidence score: |
0.8174 |