| Expression pattern: |
UP |
| Associated gene: |
Et-1, Wtap, Igf2bp2 |
| Associated microRNA: |
miR-144-3p |
| Biological function: |
promotes hepatic fibrosis, increases macrophage/KC inflammatory response and oxidative stress, and promotes HSC activation and trans-differentiation into myofibroblasts |
| Molecular mechanism: |
m6A modification by Wtap increases circDcbld2 stability via Igf2bp2 recognition; circDcbld2 acts as a ceRNA to sponge miR-144-3p and up-regulate Et-1 |
| Biological pathway or process: |
fibrosis (promotes); inflammation (promotes); oxidative stress (promotes); ceRNA regulation (promotes); HIF-1 signaling (other); NF-kappaB (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Western Blot; RIP (RNA Immunoprecipitation); MeRIP / MeRIP-seq; Luciferase Reporter Assay; Transfection; ELISA; IHC (Immunohistochemistry); IF (Immunofluorescence); RNA-seq; In Vivo Animal Model |
| Clinical significance: |
circDcbld2 was elevated in patients with HF and may act as a potential biomarker for HF treatment |
| Description: |
circDcbld2 is up-regulated in hepatic fibrosis (mouse KCs/BMDMs and human fibrotic liver) and promotes inflammation, oxidative stress, HSC activation and fibrogenesis. Wtap-mediated m6A modification and Igf2bp2 recognition increase circDcbld2 stability, and circDcbld2 sponges miR-144-3p to up-regulate Et-1. |
| Confidence score: |
0.8794 |