circRNA basic information
circBase ID: hsa_circ_0004771
Name: hsa_circ_NRIP1
Synonym: circ_0004771 / circNRIP1
Host Gene: NRIP1
Genomic location(hg19): chr21:16386664-16415895:-
Genomic location(hg38): chr21:15014343-15043574:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005075
MONDO name: thyroid gland papillary carcinoma
Disease details: papillary thyroid carcinoma / PTC
Disease DO ID:
 3969
Disease MeSH ID:
 -
Disease NCIt ID:
 C4035
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PTC tissues; adjacent normal tissues; tumor tissues
Cell lines:

Nthy-ori-1; IHH-4; SW579; TPC-1; BCPAP; K-1; HEK-293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PBX3
Associated microRNA: miR-653-5p
Biological function: promotes proliferation, invasion and migration; inhibits apoptosis; promotes tumor growth in vivo
Molecular mechanism: ceRNA mechanism: circNRIP1 sponges miR-653-5p to positively regulate PBX3 expression
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; TUNEL; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

Higher circNRIP1 expression was associated with the TNM stage and poorer overall survival.
Description:

circNRIP1 (hsa_circ_0004771) is up-regulated in papillary thyroid carcinoma and promotes malignant phenotypes (proliferation, migration/invasion, tumor growth) while inhibiting apoptosis. Mechanistically, it functions as a ceRNA by sponging miR-653-5p to de-repress PBX3, and high circNRIP1 expression correlates with advanced TNM stage and poorer overall survival.
Confidence score:

0.7944
Other information
Title:

CircNRIP1 Exerts Oncogenic Functions in Papillary Thyroid Carcinoma by Sponging miR-653-5p and Regulating PBX3 Expression.

Journal: Journal of oncology
Published: 2022
PubMed ID: 35646117
Study type:

combined biological and clinical study
Data availability: The datasets analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -