| Expression pattern: |
DN |
| Associated gene: |
STAT3 |
| Associated microRNA: |
miR-384 |
| Biological function: |
Suppresses trophoblast cell proliferation and invasion in preeclampsia. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-384, thereby modulating STAT3 expression and p-STAT3/STAT3 levels. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; Clinical Sample Validation |
| Clinical significance: |
Potential new biomarker for the diagnosis and treatment of PE. |
| Description: |
circPAPPA (hsa_circ_0088227) is down-regulated in placenta and plasma from preeclampsia patients. Functionally, reduced circPAPPA suppresses trophoblast proliferation and invasion by sponging miR-384 and decreasing STAT3/p-STAT3 via a circPAPPA-miR-384-STAT3 axis, suggesting biomarker/therapeutic potential in PE. |
| Confidence score: |
0.7968 |