circRNA basic information
circBase ID: -
Name: hsa_circ_PVT1
Synonym: circular RNA-PVT1 / circ-PVT1
Host Gene: PVT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissue; paired adjacent tissue samples; tumor tissues
Cell lines:

GES-1; HGC-27; AGS; SNu5; MKN-45
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ETS1, P21, Bcl-2, MMP9
Associated microRNA: miR-195-5p
Biological function: Circ-PVT1 promotes gastric cancer cell viability, proliferation and invasion, inhibits apoptosis, reverses the anti-GC effects of propofol, and its knockdown improves the anti-GC effect of propofol in vivo.
Molecular mechanism: Circ-PVT1 acts as a miR-195-5p sponge to modulate ETS1 expression; propofol suppresses ETS1 in GC cells through the circ-PVT1/miR-195-5p axis.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits); drug resistance (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Bioinformatics Analysis; Luciferase Reporter Assay; In Vivo Animal Model
Clinical significance:

-
Description:

Circ-PVT1 is upregulated in gastric cancer tissues and cell lines. Functionally, circ-PVT1 counteracts propofol-induced suppression of proliferation and invasion and induction of apoptosis, while circ-PVT1 knockdown enhances the anti-tumor effect of propofol in vivo. Mechanistically, circ-PVT1 sponges miR-195-5p to modulate ETS1 expression in the circ-PVT1/miR-195-5p/ETS1 axis.
Confidence score:

0.6536
Other information
Title:

Propofol suppresses gastric cancer tumorigenesis by modulating the circular RNA‑PVT1/miR‑195‑5p/E26 oncogene homolog 1 axis.

Journal: Oncology reports
Published: 2020
PubMed ID: 32945521
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -