| Expression pattern: |
UP |
| Associated gene: |
cyclin D1 |
| Associated microRNA: |
miR-195 |
| Biological function: |
Promotes LSCC cell proliferation and G1/S cell cycle transition. |
| Molecular mechanism: |
Acts as a competing endogenous RNA (ceRNA) that sponges miR-195 to increase cyclin D1 expression. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; MTT; Cell Cycle Assay; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
High circMYLK expression was closely associated with advanced clinical stage (P=0.013) of LSCC patients. |
| Description: |
circMYLK is up-regulated in LSCC tissues and cell lines and promotes LSCC cell proliferation by accelerating G1/S cell-cycle progression. Mechanistically, circMYLK functions as a ceRNA that sponges miR-195, thereby increasing cyclin D1 expression; miR-195 restoration counteracts circMYLK-driven oncogenic effects. |
| Confidence score: |
0.7129 |