| Expression pattern: |
UN |
| Associated gene: |
p38, beta-TrCP |
| Associated microRNA: |
- |
| Biological function: |
Improves ovarian function in aging mice, enhances follicular development, promotes primary granulosa cell viability, and inhibits senescence and apoptosis. |
| Molecular mechanism: |
Exosomal circHIPK3 interacts with p38 and beta-TrCP and modulates beta-TrCP-mediated ubiquitination of p38, thereby regulating MAPK signaling in primary granulosa cells. |
| Biological pathway or process: |
MAPK (other); apoptosis (inhibits); proliferation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Actinomycin D / DRB Stability Assay; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; CCK8; Annexin V/PI Flow Cytometry; TUNEL; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
- |
| Description: |
This study shows ADSC-derived exosomal circHIPK3 protects against ovarian aging by improving ovarian function and follicular development and by enhancing primary granulosa cell viability while reducing senescence and apoptosis. Mechanistically, circHIPK3 interacts with p38 and beta-TrCP and modulates beta-TrCP-mediated ubiquitination of p38, impacting MAPK signaling. |
| Confidence score: |
0.6738 |