| Expression pattern: |
UP |
| Associated gene: |
VCAM1, Ago2, RISC |
| Associated microRNA: |
miR-98-5p |
| Biological function: |
Circ-USP36 promotes ox-LDL-induced endothelial cell injury by reducing cell viability and promoting apoptosis and inflammatory responses in HUVEC cells. |
| Molecular mechanism: |
Circ-USP36 functions as a miRNA sponge for miR-98-5p, thereby increasing VCAM1 expression at the post-transcriptional level and promoting ox-LDL-induced HUVEC cell injury. |
| Biological pathway or process: |
apoptosis (promotes); inflammation (promotes); proliferation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; MTT; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation) |
| Clinical significance: |
Circ-USP36/miR-98-5p/VCAM1 axis might be an underlying therapeutic target for endothelial dysfunction in AS; clinical sample validation was proposed as a future need, not performed in this study. |
| Description: |
In an ox-LDL-induced HUVEC model of atherosclerosis-related endothelial injury, circ-USP36 is up-regulated and aggravates endothelial cell injury by inhibiting viability and promoting apoptosis and inflammation. Mechanistically, circ-USP36 sponges miR-98-5p to increase VCAM1 expression, forming a circ-USP36/miR-98-5p/VCAM1 regulatory axis that may represent a therapeutic target for endothelial dysfunction in AS. |
| Confidence score: |
0.6253 |